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KMID : 0861020170320060041
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Korea Journal of Herbology
2017 Volume.32 No. 6 p.41 ~ p.48
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The Antioxidant Activities and Neuroprotective Effects of Hot Water Extracts from Torreyae Semen
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Lee Soong-In
Choi Chan-Hun
Kim Jeong-Sang
Lim Seong-Soo
Jung Hyun-Woo
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Abstract
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Objectives : This study was designed to estimate the antioxidative and neuroprotective effects of Torreyae Semen hot water extracts (TS) .
Methods : Torreyae Semen was extracted by hot water for 2 hours with a temperature of 105 degrees. Polyphenols and total flavonoid were measured and LC-MS/MS was used to certificate anticipated antioxidative compounds. The antioxidant activities of TS were measured as scavenging effects of 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and Nitrite Oxides (NO). Cell viability and proliferation rate was measured MTT assay. The toxicities to thymocytes and splenocytes were evaluated by the proliferation rate of primary cultured cells of 7 weeks, male Balb/c mice. The antioxidant activities of TS on C6 mouse glioma cells were measured by the analysis of total glutathione contents variation. The neuroprotective effects against oxidative stresses were measured by MTT assay.
Results : Polyphenols of TS was 92.00 ± 1.24 ㎍/㎎, and total flavonoids was 0.36 ± 0.14 ㎍/㎎. TS includes gallocatechin, epigallocatechin, gallocatechin gallate and epigallocatechin gallate. TS included gallocatechin, epigallocatechin, gallocatechin gallate, epigallocatechin gallate. TS showed DPPH and NO scavenging effects as dose-dependent manner at the concentrations of 0 - 10 ㎎/㎖. In MTT assay, TS shows no significant toxicity to C6 cells, primary cultured thymocytes and splenocytes of Balb/c mice. TS increased the level of total glutathiones. TS increased cell viabilities of C6 cells against oxidative stresses such as H2O2, sodium nitroprusside (SNP), Rotenone at the concentrations of 0 - 0.063 ㎎/㎖.
Conclusions : TS shows the antioxidant and neuroprotecitive effects in these experiments.
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KEYWORD
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Torreyae Semen, Antioxidant, Neuroprotective, Hot Water Extract
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